This video demonstrates how to correctly collect and transport a nasopharyngeal (NP) aspirate for pertussis diagnostic testing. Determining who has pertussis can be difficult. Whenever possible, a properly obtained NP swab or aspirate should be collected from all persons with suspected cases. It is essential to use correct technique when collecting and transporting specimens for laboratory testing. Comments on this video are allowed in accordance with our comment policy: http://www.cdc.gov/SocialMedia/Tools/CommentPolicy.html This video can also be viewed at http://www.cdc.gov/pertussis/clinical/diagnostic-testing/specimen-collection.html#aspirate-testing